Monday, January 27, 2020

Designing a Plant Cell Model

Designing a Plant Cell Model Advanced Biology Cell Project   Ã‚   Cell Wall The cell, a complex yet basic part of our existence. Unlike humans, one of the most important parts of a cell for a plant cell is the cell wall. The cell wall gives the plant shape, strength, and enables communication to adjacent cells. This essential portion of the cell was initially found by Robert Hooke, though he named it simply wall. Other scientists, such as Karl Rudolphi and J.H.F Link, added to Hookes finding. What they found was that cells had their own individual walls, and that cells interacted through holes, later named the plasmodesmata. Before this discovery, it was thought that cell walls encircled large masses of cells, and that was the way that cells interacted. There are many different arrangements for cell walls, and certain parts (such as the secondary cell wall) do not show up in different types of cells. The most basic cell wall consists of two main layers, with the plasma membrane directly beneath it. The first layer is known as the Middle Lamella. This is the outermost layer of the cell wall. This layer mainly consists of a large amount of pectins, along with many other parts. The middle lamella is responsible for gluing the walls of two adjacent cells together, providing the stability to create plasmodesmata between the cells. The primary cell wall is mainly made up of cellulose microfibrils, which are what provide the structure and shape of the cell wall. This layer is also made up of little bits of hemicellulose, which is a polysaccharide responsible for adding extra strength to the cellulose microfibrils. If you happen to have a cell that does have a secondary cell wall, it is there to add extra rigidity and strength, so the cell likely would belong to a larger plant. This layer is primarily cellulose, lignin, glycoproteins, and polysaccharides. This layer also has three other layers (S1, S2, and S3). These layers each have more cellulose microfibrils that alternate in directions. These parts of the secondary cell wall are what allows trees to exist. As wood cell walls mainly consist of secondary cell walls, which provide the structure that they need to stand up against gravity. Cell walls are formed in an odd way. The middle lamella is laid down first, which originates from the cell plate during cytokinesis. In other words, the middle lamella is formed while the cell is splitting off from the mother cell, after the nucleus has split into two. The primary cell wall is then inserted into the middle lamella. The cellulose microfibrils, located in the primary cell wall, are created in the plasma membrane by a process known as cellulose synthase complex. This process is not fully understood, but it is thought that all cellulose-synthesizing organisms have cellulose synthase proteins, which are catalysts for the polymerization of glucan chains. This essentially all boils down to the cellulose synthase proteins kick starting the reaction that allows for the glucan chains to become cellulose. The way that I have designed my model of the cell wall, is I have spent about 5 hours going through iteration upon iteration in CAD. When I finally decided on a simplistic, yet fully informational model, I exported all the 3D models as an STL (or stereolithography file), which is a dot-matrix of the solid. My slicer software (Cura 15.04.6) then took the file, and turned it into a series of coordinates, and other commands (such as the temperature to heat up the print nozzle/bed the print speed extrusion rate). This file I then exported this file to my SD card as a .gcode file, which my 3D printer is able to understand. The SD card was then plugged into the printer, and printed. Overall, I exported 3 different .gcode files (one for the cellulose, and two others for the middle lamella and plasma membrane) as they all wouldnt fit on the build plate as a single print. All three prints took me about 4 hours combined of print time. The way that I designed my main plant cell model was similar to this, but also very different. The first thing that I had to come up with was my wiring diagram, which consisted of me figuring out what value of resistor I needed to not let the 9v battery blow out my LEDs (every color of LED requires a different value of resistor), and how I would wire everything. I then had to design the cell in CAD. The wiring diagram probably took me about an hour, as I was trying to lay everything out the most efficient way possible on a mockup of my proto-board (done in circuit design software). I then spent about 10 hours total designing each individual part in CAD (I also ordered all the parts and got them 2 day shipped). Once all the parts arrived, I was just finishing up with the CAD, so I started the prints on the parts (the base board alone took 3 hours as I set it to a higher infill (50% instead of 10%) as I wanted it to be more substantial, and sturdy. The other parts took about 4 and a ha lf more hours to print (I printed excess of the organelles in case one broke while drilling). Bibliography Cell Wall. Wikipedia. Wikimedia Foundation, 18 Feb. 2017. Web. 19 Feb. 2017. Cell Wall. Cell Wall Biology-Online Dictionary. Web. 19 Feb. 2017. Cellulose Synthase (UDP-forming). Wikipedia. Wikimedia Foundation, 19 Feb. 2017. Web. 19 Feb. 2017. Cytokinesis. Wikipedia. Wikimedia Foundation, 01 Feb. 2017. Web. 19 Feb. 2017. Li, Shundai, Logan Bashline, Lei Lei, and Ying Gu. Cellulose Synthesis and Its Regulation. The Arabidopsis Book / American Society of Plant Biologists. American Society of Plant Biologists, 2014. Web. 19 Feb. 2017.

Sunday, January 19, 2020

Frankenstein :: Essays Papers

Frankenstein Protagonist: The protagonist in the novel is Victor Frankenstein. He is the main character who contends with the conflict in the novel. His decision to create life provides a problem that he attempts to escape but eventually marks his death. Antagonist: The antagonist in the novel is also the protagonist, Victor Frankenstein. Victor may have directed all of his hate and blame towards the monster he created, but is worst enemy lay within himself and his refusal to accept responsibility for his actions. Conflict: The main conflict in the novel is based on the â€Å"monster† Victor Frankenstein created in his laboratory. He neglects his responsibility to the monster he created by ignoring its existence, and his cowardice leads to inner feelings of guilt and unhappiness that ultimately cause his life to unravel and the people he loves to perish. His refusal to be accountable for his action brings the misery and misfortune that constitute the foundation of the novel. Chronology: Robert Walton writes in his first letter to his sister Margaret Saville about his desire to explore the world. His second letter then tells about his preparations for a crew and more about how he desires to explore the unexplored. In this letter he also explains how he wishes he had a friend to share his life with. In his third letter, he tells how the voyage is underway and going well. His fourth letter tells how the ship became trapped between floating blocks of ice and, after being freed, the ship encounters and takes aboard a man who was stranded on floating ice. Walton tells how the man is in wretched condition and is very melancholy. He tells how he gradually befriends the man and, after talking for some time, the man agrees to tell Walton the story of his life and how he came to be where he is now. His name is told to be Victor Frankenstein. Walton, during the narration, takes notes in the form of a letter for Margaraet. Victor Frankenstein was born in Geneva, Switzerland, to a family of notoriety. His family adopted a young girl his age named Elizabeth from a poor family who could not support her. As a child, Victor was fascinated with alchemy and sciences. At age seventeen, Victor’s mother died.

Saturday, January 11, 2020

Corporate Law- Promoter Essay

Rolly approached Molly and Polly and asked them to be shareholders to form a company. Then Rolly acquired a shop lot worth RM350,000 in order to prepare for the company in future. She bought this shop lot below the market price as the vendor was looking urgently to sell it. After the company is formed, she sold the shop lot to Jiggles Sdn. Bhd. at market price. Thus, she gained a lucrative profit of RM200,000 without the knowledge of any other party. Definition of promoter Section 4(1) of the Companies Act 1965 defined promoter is a person preparing prospectus. (not limited to this definition alone) According to Cockburn CJ in Twycross v Grant (1877), a promoter is a â€Å"person who undertakes to form a company with reference to a given object and set it going and takes the necessary steps to accomplish the purpose†. (Company Law in Malaysia, pg42) A promoter may be a natural person for example when the sole proprietor promotes a company to take over his business. (Company Law in Malaysia, pg42) The promotion process includes negotiations, registration of the company, obtaining directors and shareholders and preparing the paper work. In this case, Rolly is the promoter of Jiggles Sdn. Bhd as she meets the definition of promoters. She is a sole proprietor who selling cupcakes, promoted the company to take over her business. Besides, she also obtained shareholders by approaching Molly and Polly. Promoters’ duties Thus, Rolly is under a fiduciary relationship with the company. She has an obligation to act in good faith for the best interest of the company and must avoid conflict of interest which will affect her behavior. Under the duty of promoter, promoter should not make secret profit, defraud the company by active concealment, disclose confidential information, hides any personal interest. On the other hand, they should make a true and honest account to the company for his dealing on behalf of the company and make full and frank disclosure to shareholders and directors. In this case, Rolly had breached her fiduciary duty. She had made a secret profit of RM200,000. She did not make full and frank disclosure of her interest to the shareholders and directors of the company. She had hide her personal interest. Thus, where Rolly fails to declare her interest in the contract with the company she is promoting, the contract is voidable at the company’s  option as in the case of Erlanger v New Sombrero Phosphate Co (1878). Remedies of breach of duty As in the case of Erlanger v New Sombrero Phosphate Co (1878), the contract is voidable at the company’s option. Thus, the company may avail itself of the following remedies. The company may rescind the contract, claim for damages or recovery of secret profit. Rescind the contract-The company may choose to put an end to the contract. If the company terminates the contract, both parties are to return the benefits received. Thus, the company has to return the shop lot and Rolly has to return the purchase price received. Damages- The court may order Rolly to pay damages to the company for loss incurred when rescission is not possible, as in the case of Re Leeds and Handley Treatres of Variety. Recovery of secret profits- When rescission is not possible or the company does not want to rescind the contract, the company can recover the secret profit made by Rolly.